Charge Variant Analysis of Trastuzumab using a bioZen 6 µm WCX Column with a pH Gradient and Native MS Detection

Abstract:

In this application note we show the separation of charge variants of Trastuzumab under native conditions using a pH gradient formed with volatile buffers. The novel, MS-friendly buffers used for the pH gradient are stable for at least one week at room temperature when an inlet air filter is used (ex. SecurityCAP™) on the reservoir.

Document Type:

Technical Notes

Industries:

Pharmaceutical/Biopharmaceutical

Techniques:

HPLC / UHPLC

Brands:

bioZen

PDF Link:

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